Neural Stem Cell Metabolic Changes in the Presence of Interferon-gamma, Is A Well-Researched Topic, It Is To Be Used As A Guide Or Framework For Your Research.
The spinal cord serves as the major connection between the brain and other nerves distributed throughout the body. Injury to spinal cord tissue can be caused by genetic disorders, infections, and trauma that results in a loss of connectivity that leads to clinical disability. Repair of the injured spinal cord is difficult because the CNS has limited regenerative capacity1. One therapeutic option for spinal cord injury is the replacement of damaged cells by stem cells1. Neural stem cells (NSCs) are defined as cells in the human nervous system that can develop into any type of neuron or glial cell after forming an intermediate precursor cell2. Previous work has identified the cytokine interferon-gamma (IFN-γ) as a stimulatory molecule that promotes the differentiation of NSCs to mature neurons3. One potential method to expose NSCs to IFN-γ is to seed them onto a biocompatible hydrogel composed of modified chitosan (Figure 1).
In this experiment, two scientific questions will be explored: What specific metabolic pathways are activated to guide neural stem cell differentiation after NSCs are exposed to IFN-γ? Is oxidative phosphorylation used by neural stem cells as an energy requirement after they are exposed to IFN-γ? In order to examine the metabolism of NSCs, global metabolomic profiling and immunohistochemistry will be used to track biochemical changes and protein migrations in the cells as they are exposed to IFN-γ5,6